The following standards establish the specifications for biofuels in the European Union:
- EN 14214 includes specifications for fatty acid methyl ester (FAME) fuel for diesel engines. B100 that meets this standard could be used unblended in a diesel engine (if the engine has been adapted to operate on B100) or blended with petroleum diesel fuel.
- EN 590, the European diesel fuel specification, is also applicable to biodiesel blends up to 7% of FAME.
- EN 15376 establishes specification for ethanol to be blending into gasoline in the EU.
- EN 228, the European gasoline fuel specification, is also applicable to ethanol blends up to 5%.